29, నవంబర్ 2011, మంగళవారం

Nikon Small World 2011 competition winners: photographs through the microscope


1st Place: Dr. Igor Siwanowicz, Max Planck Institute of Neurobiology, Martinsried, Germany. Portrait of a Chrysopa sp. (green lacewing) larva (20x), Confocal
The winners of the 2011 Nikon Small World Photomicrography Competition have been announced. This year's top honours go to Dr Igor Siwanowicz with a micrograph that demonstrates the beauty in "ugly". When a small bug landed on Dr Siwanowicz’s hand and began digging its mandibles into his skin, he didn't swat it away. Instead, he removed a tiny test tube from his pocket – which he carries for occasions such as these – and captured it as a potential subject for his photomicrography passion. This chance meeting with a Common Green Lacewing led to him being named the winner of the 2011 Nikon Small World competition.
Now in its 37th year, the contest recognises excellence in photomicrography, honouring images that successfully showcase the delicate balance between scientific technique and artistic quality. A full gallery of winning images, along with Images of Distinction can be viewed at www.nikonsmallworld.com
1st Place: Dr. Igor Siwanowicz, Max Planck Institute of Neurobiology, Martinsried, Germany. Portrait of a Chrysopa sp. (green lacewing) larva (20x), Confocal


2nd Place: Dr. Donna Stolz, University of Pittsburgh
Pittsburgh, Pennsylvania, USA. Blade of Grass (200X), 
Confocal stack reconstruction, Autofluorescence
2nd Place: Dr. Donna Stolz, University of Pittsburgh Pittsburgh, Pennsylvania, USA. Blade of Grass (200X), Confocal stack reconstruction, Autofluorescence


3rd Place: Frank Fox, Fachhochschule Trier, Trier, Rheinland-Pfalz, Germany. Melosira moniliformis, living specimen (320X), Differential Interference Contrast
3rd Place: Frank Fox, Fachhochschule Trier, Trier, Rheinland-Pfalz, Germany. Melosira moniliformis, living specimen (320X), Differential Interference Contrast


4th Place: Dr. Robin Young, The University of British Columbia, Vancouver, British Columbia, Canada. Intrinsic fluorescence in Lepidozia reptans (liverwort) (20X), 
Live mount, Confocal microscopy
4th Place: Dr. Robin Young, The University of British Columbia, Vancouver, British Columbia, Canada. Intrinsic fluorescence in Lepidozia reptans (liverwort) (20X), Live mount, Confocal microscopy
Picture: Nikon Small World / 


5th Place: Alfred Pasieka, Germany. Microchip surface, 3D reconstruction (500X), Incident light, Normarski Interference Contrast
5th Place: Alfred Pasieka, Germany. Microchip surface, 3D reconstruction (500X), Incident light, Normarski Interference Contrast


7th Place: Gabriel Luna, UC Santa Barbara, Neuroscience Research Institute, Santa Barbara, California, USA. Retinal flatmount of mouse nerve fiber layer (40X), Laser Confocal Scanning
7th Place: Gabriel Luna, UC Santa Barbara, Neuroscience Research Institute, Santa Barbara, California, USA. Retinal flatmount of mouse nerve fiber layer (40X), Laser Confocal Scanning


8th Place: Dr. Bernardo Cesare, Department of Geosciences
Padova, Italy. Graphite-bearing granulite from Kerala (India) (2.5X), Polarised light
8th Place: Dr. Bernardo Cesare, Department of Geosciences Padova, Italy. Graphite-bearing granulite from Kerala (India) (2.5X), Polarised light


9th Place: Dr. Jan Michels, Christian-Albrechts-Universität zu Kiel, Kiel, Germany. Temora longicornis (marine copepod), ventral view (10X), Confocal, Autofluorescence and Congo Red Fluorescence
9th Place: Dr. Jan Michels, Christian-Albrechts-Universität zu Kiel, Kiel, Germany. Temora longicornis (marine copepod), ventral view (10X), Confocal, Autofluorescence and Congo Red Fluorescence


10th Place: Joan Röhl, Institute for Biochemistry and Biology
Potsdam, Germany. Daphnia magna (freshwater water flea) (100X), Differential Interference Contrast
10th Place: Joan Röhl, Institute for Biochemistry and Biology Potsdam, Germany. Daphnia magna (freshwater water flea) (100X), Differential Interference Contrast


11th Place: Dr. Jan Michels, Christian-Albrechts-Universität zu Kiel, Kiel, Germany. Ant head, frontal view (10X), 
Confocal, autofluorescence
11th Place: Dr. Jan Michels, Christian-Albrechts-Universität zu Kiel, Kiel, Germany. Ant head, frontal view (10X), Confocal, autofluorescence


12th Place: Thomas Deerinck, National Center for Microscopy and Imaging Research, La Jolla, California, USA. HeLa (cancer) cells (300X), 2-Photon fluorescence
12th Place: Thomas Deerinck, National Center for Microscopy and Imaging Research, La Jolla, California, USA. HeLa (cancer) cells (300X), 2-Photon fluorescence
13th Place: Dr. Stephen S. Nagy, Montana Diatoms
Helena, Montana, USA. Curare vine in cross-section, Chondrodendron tomentosum (45X), Brightfield, digitally inverted
13th Place: Dr. Stephen S. Nagy, Montana Diatoms Helena, Montana, USA. Curare vine in cross-section, Chondrodendron tomentosum (45X), Brightfield, digitally inverted
14th Place: Yanping Wang, Beijing Planetarium, Beijing, China. Sand (4X), Reflected light
14th Place: Yanping Wang, Beijing Planetarium, Beijing, China. Sand (4X), Reflected light
15th Place: James H. Nicholson, Coral Culture and Collaborative Research Facility, NOAA/NOS/NCCOS/CCEHBR & HML
Charleston, South Carolina, USA. Porites lobata (lobe coral), live specimen displaying tissue pigmentation response with red fluorescence (12X), Epifluorescence with triple band (U/B/G) excitation
15th Place: James H. Nicholson, Coral Culture and Collaborative Research Facility, NOAA/NOS/NCCOS/CCEHBR & HML Charleston, South Carolina, USA. Porites lobata (lobe coral), live specimen displaying tissue pigmentation response with red fluorescence (12X), Epifluorescence with triple band (U/B/G)
16th Place: Dr. Christopher Guérin, VIB (Flanders Institute of Biotechnology), Ghent, Belgium. Cultured cells growing on a bio-polymer scaffold (63X), Confocal
16th Place: Dr. Christopher Guérin, VIB (Flanders Institute of Biotechnology), Ghent, Belgium. Cultured cells growing on a bio-polymer scaffold (63X), Confocal
17th Place: Dr. Witold Kilarski, EPFL-Laboratory of Lymphatic and Cancer Bioengineering, Lausanne, Switzerland. Litomosoides sigmodontis (filaria worms) inside lymphatic vessels of the mouse ear (150X), Fluorescent confocal microscopy
17th Place: Dr. Witold Kilarski, EPFL-Laboratory of Lymphatic and Cancer Bioengineering, Lausanne, Switzerland. Litomosoides sigmodontis (filaria worms) inside lymphatic vessels of the mouse ear (150X), Fluorescent confocal microscopy
18th Place: Benjamin Blonder, David Elliott, University of Arizona, Tucson, Arizona, USA. Venation network of young Populus tremuloides (quaking aspen) leaf (4X), 
Brightfield image of safranin-stained tissue
18th Place: Benjamin Blonder, David Elliott, University of Arizona, Tucson, Arizona, USA. Venation network of young Populus tremuloides (quaking aspen) leaf (4X), Brightfield image of safranin-stained tissue
19th Place: Dr. Donna Stolz, The University of Pittsburgh
Pittsburgh, Pennsylvania, USA. Mammalian cell collage stained for various proteins and organelles, assembled into a wreath (200-2000X), Single slice confocal cell mosaic
19th Place: Dr. Donna Stolz, The University of Pittsburgh Pittsburgh, Pennsylvania, USA. Mammalian cell collage stained for various proteins and organelles, assembled into a wreath (200-2000X), Single slice confocal cell mosaic
20th Place: Douglas Moore, University of Wisconsin - Stevens Point, Stevens Point, Wisconsin, USA. Agatised dinosaur bone cells, unpolished, ca. 150 million years old (42X), 
Stereomicroscopy, fiber optics
20th Place: Douglas Moore, University of Wisconsin - Stevens Point, Stevens Point, Wisconsin, USA. Agatised dinosaur bone cells, unpolished, ca. 150 million years old (42X), Stereomicroscopy, fiber optics

Dr. John H Brackenbury, University of Cambridge, UK: 
Water droplet containing a pair of mosquito larvae
(Laser-triggered high-speed macrophotography)
We end this gallery with a selection of the pictures that were named as Images of Distinction by the competition judges. Dr John H Brackenbury, University of Cambridge, UK: Water droplet containing a pair of mosquito larvae (Laser-triggered high-speed macrophotography)

Walter Piorkowski, South Beloit, Illinois. Jumping spider anterior lateral and median eyes, Reflected light
Walter Piorkowski, South Beloit, Illinois. Jumping spider anterior lateral and median eyes, Reflected light
Dr. Willard Strong, Microsphere Images, Oyama, Canada.
Swallowtail butterfly (family Papilionidae) pupal stage chrysalis, tethered to its pupal home, Stereomicroscopy
Dr. Willard Strong, Microsphere Images, Oyama, Canada. Swallowtail butterfly (family Papilionidae) pupal stage chrysalis, tethered to its pupal home, Stereomicroscopy
Wim van Egmond, Micropolitan Museum, Rotterdam, Netherlands. Leptodora kindtii (giant waterflea) eye; living specimen, Differential Interference Contrast
Wim van Egmond, Micropolitan Museum, Rotterdam, Netherlands. Leptodora kindtii (giant waterflea) eye; living specimen, Differential Interference Contrast

Jose R. Almodovar, Microscopy Center, Biology Department, UPR Mayaguez Campus, Mayaguez, Puerto Rico. Fresh water shrimp eye and head, Image Stacking Photography
Jose R. Almodovar, Microscopy Center, Biology Department, UPR Mayaguez Campus, Mayaguez, Puerto Rico. Fresh water shrimp eye and head, Image Stacking Photography

Charles Krebs, Charles Krebs Photography, Issaquah, Washington, USA. Corixidae sp. (water boatman), 
Reflected light
Charles Krebs, Charles Krebs Photography, Issaquah, Washington, USA. Corixidae sp. (water boatman), Reflected light
Edwin Lee, Carrollton, Texas, USA. Lamium sp. (Henbit deadnettle) stamens and petal, wet mount, Polarised light
Edwin Lee, Carrollton, Texas, USA. Lamium sp. (Henbit deadnettle) stamens and petal, wet mount, Polarised light
 
Dr. Douglas Clark, Paedia Corporation, San Francisco, California. Cethosia biblis (butterfly) dried wing scales,
Incandescent, darkfield
Dr. Douglas Clark, Paedia Corporation, San Francisco, California. Cethosia biblis (butterfly) dried wing scales, Incandescent, darkfield
Jesper Grønne, Photographer Groenne.eu, Silkeborg, Denmark. Natural formed frost crystal. Grew overnight on a fence in -15 degrees C, No special technique
Jesper Grønne, Photographer Groenne.eu, Silkeborg, Denmark. Natural formed frost crystal. Grew overnight on a fence in -15 degrees C, No special technique
Wolfgang Bettighofer, Kiel, Germany. Micrasterias apiculata (green alga) cell wall, living specimen, Differential Interference Contrast
Wolfgang Bettighofer, Kiel, Germany. Micrasterias apiculata (green alga) cell wall, living specimen, Differential Interference Contrast
John Gaynes, University of Utah, Salt Lake City, Utah. 
3 day post-fertilization zebrafish embryo, Confocal
John Gaynes, University of Utah, Salt Lake City, Utah. 3 day post-fertilization zebrafish embryo, Confocal
Dr. Terue Kihara, German Centre for Marine Biodiversity Research (DZMB) - Seckenberg am Meer, Germany. 
Clausidium sp. nov., female with egg sacs, ventral view, 
Confocal
Dr. Terue Kihara, German Centre for Marine Biodiversity Research (DZMB) - Seckenberg am Meer, Germany. Clausidium sp. nov., female with egg sacs, ventral view, Confocal
David Millard, Austin, Texas, USA. Fire agate, 
Diffuse incident illumination
David Millard, Austin, Texas, USA. Fire agate, Diffuse incident illumination
Dr. Gregor Overney, Agilent Technologies, Santa Clara, California, USA. 20GB hard disk read/write head slider, 
Episcopic oblique brightfield
Dr. Gregor Overney, Agilent Technologies, Santa Clara, California, USA. 20GB hard disk read/write head slider, Episcopic oblique brightfield
Haris Antonopoulos, Athens, Greece. DVD-ROM front lens and part of movement coil, Reflected and transmitted light
Haris Antonopoulos, Athens, Greece. DVD-ROM front lens and part of movement coil, Reflected and transmitted light
Dr. Michael M. Raith, Steinmann Institut, University of Bonn
Bonn, Germany. Leucite crystal from volcanic rock showing polysynthetic lamellar twins formed by the cubic- tetrago,
Transmitted polarised light, crossed polarisation
Dr. Michael M. Raith, Steinmann Institut, University of Bonn Bonn, Germany. Leucite crystal from volcanic rock showing polysynthetic lamellar twins formed by the cubic- tetrago, Transmitted polarised light, crossed polarisation
Dr. Giorgio Seano and Dr. Roberto Sessa, Institute for Cancer Research and Treatment, Candiolo (Torino), Italy. 
Zebrafish embryos (Dario rerio) vascular system at seven days post-fertilisation, Confocal
Dr. Giorgio Seano and Dr. Roberto Sessa, Institute for Cancer Research and Treatment, Candiolo (Torino), Italy. Zebrafish embryos (Dario rerio) vascular system at seven days post-fertilisation, Confocal
Wim van Egmond, Micropolitan Museum, Rotterdam, Netherlands. Young Volvox sp. (green algae) colony, 
Differential Interference Contrast
Wim van Egmond, Micropolitan Museum, Rotterdam, Netherlands. Young Volvox sp. (green algae) colony, Differential Interference Contrast
Dr David Maitland (www.davidmaitland.com), Feltwell, UK: Double compound eyes of male Bibio marci. Larger - Reflected (Episcopic), Diffuse Illumination
Dr David Maitland (www.davidmaitland.com), Feltwell, UK: Double compound eyes of male Bibio marci. Larger - Reflected (Episcopic), Diffuse Illumination
David Millard, Austin, Texas: Vanessa atalanta (Red admiral butterfly) egg in Urtica dioica (Stinging nettle) - trichomesal
David Millard, Austin, Texas: Vanessa atalanta (Red admiral butterfly) egg in Urtica dioica (Stinging nettle) - trichomesal
Pekka Honkakoski, Iisalmi, Finland: Rare column snowflake with thin, knifelike ice extensions (Triangle brightfield, oblique blue, opposite oblique red)
Pekka Honkakoski, Iisalmi, Finland: Rare column snowflake with thin, knifelike ice extensions (Triangle brightfield, oblique blue, opposite oblique red)


Debora Leite, University of Sao Paulo: Sugarcane root cross section
Debora Leite, University of Sao Paulo: Sugarcane root cross section

Stephen S Nagy, MD, Montana Diatoms, Helena, Montana: Tip of a Butterfly tongue (Polarized light, brightfield)
Stephen S Nagy, MD, Montana Diatoms, Helena, Montana: Tip of a Butterfly tongue (Polarised light, brightfield)
Dr Havi Sarfaty, Israeli Veterinary Association, Ramat-Gan, Israel: Mouth of common fly (Fiber optic illumination)
Dr Havi Sarfaty, Israeli Veterinary Association, Ramat-Gan, Israel: Mouth of common fly (Fiber optic illumination)


Marc van Hove, Centexbel, Merelbeke, Belgium: 
Tufting needle eye with yarn (Epi-brightfield)
Marc van Hove, Centexbel, Merelbeke, Belgium: Tufting needle eye with yarn (Epi-brightfield)

Arik Shapira, Hod HaSharon, Israel: Internal parts of a wildflower
Arik Shapira, Hod HaSharon, Israel: Internal parts of a wildflower
Poulomi Ray, Department of Biological Sciences, Clemson University, Clemson, South Carolina, USA: Chick embryo intestine (20X) - Confocal
Poulomi Ray, Department of Biological Sciences, Clemson University, Clemson, South Carolina, USA: Chick embryo intestine (20X) - Confocal

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